RNA interference-mediated functional characterization of Group I chitin deacetylases in Holotrichia parallela Motschulsky
نویسندگان
چکیده
منابع مشابه
Extraction and characterization of chitin from the beetle Holotrichia parallela Motschulsky.
Insect chitin was isolated from adult Holotrichia parallela by treatment with 1 M HCl and 1 M NaOH, following by 1% potassium permanganate solution for decolorization. The yield of chitin from this species is 15%. This insect chitin was compared with the commercial a-chitin from shrimp, by infrared spectroscopy, X-ray diffraction, scanning electron microscopy, and elemental analysis. Both chiti...
متن کاملIdentification of a new peritrophic membrane protein from larval Holotrichia parallela (Coleoptera: Motschulsky).
Peritrophic membranes (PMs) are composed of proteins, proteoglycans and chitin that play important roles in the structural formation and function of the PM. This study identified and characterized a new chitin binding protein named HpCBP45 by immunoscreening of the Holotrichia parallela larvae midgut expression library. The predicted amino acid sequence indicates that it contains eight tandem ch...
متن کاملConstruction and Characterization of a Cellulolytic Consortium Enriched from the Hindgut of Holotrichia parallela Larvae
Degradation of rice straw by cooperative microbial activities is at present the most attractive alternative to fuels and provides a basis for biomass conversion. The use of microbial consortia in the biodegradation of lignocelluloses could reduce problems such as incomplete synergistic enzymes, end-product inhibition, and so on. In this study, a cellulolytic microbial consortium was enriched fr...
متن کاملFunctional characterization of three trehalase genes regulating the chitin metabolism pathway in rice brown planthopper using RNA interference
RNA interference (RNAi) is an effective gene-silencing tool, and double stranded RNA (dsRNA) is considered a powerful strategy for gene function studies in insects. In the present study, we aimed to investigate the function of trehalase (TRE) genes (TRE 1-1, TRE 1-2, and TRE-2) isolated from the brown planthopper Nilaparvata lugens, a typical piercing-sucking insect in rice, and investigate the...
متن کاملHigh Genetic Diversity of Microbial Cellulase and Hemicellulase Genes in the Hindgut of Holotrichia parallela Larvae
In this study, we used a culture-independent method based on library construction and sequencing to analyze the genetic diversity of the cellulase and hemicellulase genes of the bacterial community resident in the hindgut of Holotrichia parallela larvae. The results indicate that there is a large, diverse set of bacterial genes encoding lignocellulose hydrolysis enzymes in the hindgut of H. par...
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ژورنال
عنوان ژورنال: Pesticide Biochemistry and Physiology
سال: 2021
ISSN: 0048-3575
DOI: 10.1016/j.pestbp.2021.104770